The content is provided for information purposes only. In order to create these cell lines, the genetic make-up of the cells has to be fiddled with to make them immortal and keep them alive in the petri dish, Dr. David Strain, a senior clinical lecturer at Englands University of Exeter Medical School, wrote in an email to the AP. Moore also cautioned that the cells used in the study were quite different than primary cells in the body. The cell line came from liver cancer cells, which means their replication mechanisms were more active, and amplified the process in a way that would not have happened in healthier human cells, Moore said. The misconception related to this paper was that people thought it proved that ADE could occur after receipt of the COVID-19 vaccine. The Swedish study also found spike proteins expressed on the surface of the liver cells that researchers say may be targeted by the immune system and possibly cause autoimmune hepatitis, as there [have] been case reports on individuals who developed autoimmune hepatitis after BNT162b2 vaccination.. Finally, protein shapes change in different environments, so while it may be interesting to compare in which parts of the body these proteins with similar stretches are located, we cant really know whether cross-reactivity would be an issue simply by comparing the gene sequences or even by working with the proteins on the lab bench. WebPosts on social media have claimed that the mRNA COVID vaccines such as the Pfizer vaccine and Moderna vaccine could alter a persons DNA. Likewise, the Results section has five sentences. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.5-2% of the injection site concentration. The Japanese regulatory agencys biodistribution study (pdf) of the Pfizer vaccine showed that some of the mRNAs moved from the injection site and through the bloodstream, and were found in various organs such as the liver, spleen, adrenal glands, and ovaries of rats 48 hours following injection. Philadelphia, PA 19104, Know My Rights About Surprise Medical Bills. Drugs and Lactation Database (LactMed) [Internet]. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.\r\rURL list from Monday, Feb. 28 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rLINE-1 protein localization and functional dynamics during the cell cycle | eLife\rhttps://elifesciences.org/articles/30058\r\rTime evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv\rhttps://www.biorxiv.org/content/10.1101/2021.08.20.457104v1\r\rReverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed\rhttps://pubmed.ncbi.nlm.nih.gov/33958444/\r\rNo evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC\rhttps://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/\r\rOverview of Reverse Transcription - Retroviruses - NCBI Bookshelf\rhttps://www.ncbi.nlm.nih.gov/books/NBK19424/\r\rNuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology\rhttps://www.nature.com/articles/s41564-020-0735-8\r\rChimeric RNA - Wikipedia\rhttps://en.wikipedia.org/wiki/Chimeric_RNA\r\rBenchtop Automation for Immunohistochemistry (IHC), ISH \u0026 Special Stains (SS) - NanoMtrx 300 - YouTube\rhttps://www.youtube.com/watch?v=3IRl0kbR9-o\u0026ab_channel=BioGenexLaboratories\r\rSchrdinger's cat - Wikipedia\rhttps://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the A new Swedish study published in MDPI found that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact Another aspect of protein chemistry is also important. Clin Microbiol Infect. Keywords: The messenger RNA (mRNA) fromPfizersCOVID-19vaccine is able to enter humanlivercells and is converted intoDNA, according to Swedish researchers at Lund University. Please enable it to take advantage of the complete set of features! Spetzofai is a rustic spicy Greek dish that is made with thick sausages and long green and red peppers in a rich tomato sauce. About this website If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. This paper compared protein sequences from the SARS-CoV-2 virus to protein sequences in humans and described where the latter are found in the body. Social media users are falsely claiming that a Swedish study conducted by Lund University found that mRNA COVID-19 vaccines permanently alter human DNA. Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. A gene that the researchers studied produces the protein LINE-1. In fact. On the figure, the two lines (comparing vaccinated and unvaccinated patient data) are almost in the same place for the first four or five days of illness, but then they separate, and the vaccinated group more quickly requires higher numbers of PCR cycles to detect viral RNA, meaning there was less virus in those samples. And, this month, we saw a few of the many ways that science can be misinterpreted and misused. Cells that received no vaccine at all were used for control purposes.The researchers then investigated how different gene expressions in the cells changed over time. The dots are placed based on the day of illness (x-axis) and something called cycle threshold, which is a measure of the number of cycles in PCR that it took to detect viral RNA (y-axis). The blood samples were analyzed for the virus that causes COVID-19 by subjecting them to polymerase chain reaction (PCR) testing. The Vaccine Education Center staff regularly reviews materials for accuracy. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. Get weekly and/or daily updates delivered to your inbox. Kids will surely love it Serve with crusty bread! BNT162b2 mRNA is reverse transcribed intracellularly into DNA as fast as 6 [hours] after BNT162b2 exposure.. It is reverse transcribed by LINE-1 proteins of the cells. HHS Vulnerability Disclosure, Help The spike protein naturally occurs on the outside of the virus that causes Covid. About BVHS; Mayo Clinic Care Network; News & Media; Contact Us BNT: BNT162b2; L: DNA ladder; Ctrl1: cultured Huh7 cells; Ctrl2: Huh7 cells without BNT162b2 treatment collected at 6 h; Ctrl3: Huh7 cells without BNT162b2 treatment collected at 24 h; Ctrl4: Huh7 cells without BNT162b2 treatment collected at 48 h; Ctrl5: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h; Ctrl6: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h, digested with RNase. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. Thank you for taking time to provide your feedback to the editors. Anti-vaccination and ivermectin proponents have gone bananas over the new study from the Lund University and transmitted securely. 2022;44(3):1115-26. By using our site, you acknowledge that you have read and understand our Privacy Policy Its precisely what health experts and fact-checkers said for more than a year could not occur. Experts, and the study authors, say the research is being misinterpreted. Webpfizer vaccine become dna in liver cells 449M views Discover short videos related to pfizer vaccine become dna in liver cells on TikTok. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. It is a good tool when studying molecular and cellular processes, they are easy to research, and since the cell lines are easily accessible, studies often start with various cell lines. Data, especially data from vaccinated humans, will hopefully sort out the question marks. Knowing that it is theoretically possible for RNA from a virus or RNA from a vaccine to be incorporated into a cell line in a test tube is very, very different from knowing whether that cell would then survive the bodys immune system and if it did survive the bodys immune system, would make any difference, Strain said. Mandatory vaccinations, the segregation of citizens, and the promotion of inequality in the modern democracy of Greece and other democratic countries in the era of COVID-19. And, while this may be the case early during infection, there are a few additional considerations when thinking about these data: Hopefully these examples demonstrated some of the ways that a paper should be examined. The whole process occurred rapidly within six hours. Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. But studies are showing that is not the case. Skip to main content. Coronaviruses, however, are not expected to do this, said Bethany Moore, chair of the University of Michigans Department of Microbiology and Immunology. However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. Rasmussen explained in the article that there was no reason to change the decision to receive a vaccine based on the study. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. Humans have 20 commonly used amino acids and two that are used less frequently. Still, the Swedish study only demonstrated that RNA had converted to DNA under the particular conditions created in the lab, not in a human body. The .gov means its official. Your feedback is important to us. Dr. Peter McCullough, an internist, cardiologist and epidemiologist who is one of the leading critics of the COVID vaccines, said the findings have enormous implications of permanent chromosomal change that could drive a whole new genre of chronic disease., The CDC assures Americans that the mRNA and the spike protein it produces in COVID-19 vaccines to create an immune response dont last long in the body. On its website, the agency states: Our cells break down mRNA and get rid of it within a few days after vaccination. Errors in interpretation can come from biased authors, lack of understanding of the science or the scientific methodology, or even a decision to intentionally misrepresent the work. Epub 2021 Nov 10. FOIA Disclaimer. Reverse transcription is when DNA is made from RNA, whereas the normal transcription process involves a portion of the DNA serving as a template to make an mRNA molecule inside the nucleus. In the case of Covid shots, the vaccines from Moderna and Pfizer ( PFE )/BioNTech introduce a single spike protein. Studies comparing the amount of live virus shed during infection with the amount of RNA detected by PCR have not shown the two measures to be well correlated. As such, finding similar stretches of genes is not novel, unlikely, or particularly surprising. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Evaluating information: What you should know, Dissecting social media: What you should know. 2021 Jun 7;22(5):172. doi: 10.1208/s12249-021-02058-y. We show that the vaccine enters liver cells as early as 6 hours after the vaccine has been administered. Drugs and vaccines used for treating and preventing coronavirus infection also have hepatotoxicity. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: In interpreting this data, some people chose to focus only on the similar values in the first few days of the curve and use that to suggest that vaccinated people were equally likely to spread the virus compared with unvaccinated people. Why liver cells and why the specific dose? 5. This has been the question our study aims to answer. The authors said more investigations are needed, including in whole living organisms such as animals, to better understand the potential effects of the mRNA vaccine. The persistence of the spike protein in the body raises the prospect of sustained inflammation within and damage to organs which express the spike protein, according to experts atDoctors for COVID Ethics,an organization consisting of physicians and scientists seeking to uphold medical ethics, patient safety, and human rights in response to COVID-19.. https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support). No added sugar, full of protein and chocolate! eCollection 2022. 2022 Dec 9;14(12):e32361. Based on this study, is there any reason to not get vaccinated? Accessibility statement. Differences in mRNA-1273 (Moderna) and BNT162b2 (Pfizer-BioNTech) SARS-CoV-2 vaccine immunogenicity among patients undergoing dialysis. Some of the vaccine dose is going to make it into the bloodstream, of course. Suggested accounts. One of the limitations of our study is that we dont know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies, de Marinis said. In a lab environment, i.e., in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumor. Curr Issues Mol Biol. The text in one post reads: BOMBSHELL: Biological Study PROVES Pfizer mRNA Vaccine Permanently Alters Human DNA. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 Front Immunol. Shakira. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. Webhttps://lnkd.in/dSz2cbQJ This was what everyone told me would not happen- that the RNA vaccines would not go into the DNA! The messenger RNA (mRNA) from Pfizer s COVID-19 or, by Lund University. Giving to Lund Pfizers BNT162b2 mRNA Covid-19 vaccine was the first vaccine against the novel coronavirus (COVID-19) approved for distribution in the United States. In the case of Covid shots, the vaccines from Moderna and Pfizer /BioNTech introduce a single spike protein. Current Issues in Molecular Biology. 5. 41.1M. The paper does not provide such evidence. CLAIM: A Swedish study shows that Pfizer's COVID-19 vaccine changes recipients' DNA. First, cancerous cell lines replicate, whereas our liver cells typically are not replicating. A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. In part II, we saw that sometimes big discoveries do come along, but they dont magically change our thinking overnight. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. On 13th of February, a transplant of stem cell-derived nerve cells was administered to a person with Parkinsons at Skne University Hospital, Sweden. RNA levels of BNT162b2 are presented as logged 2, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. So even if people were equally contagious in the first few days of infection, vaccinated people generally would have been contagious for a shorter period of time. Salesforce has already slashed 8,000 workers from its ranks this year. For these reasons, while the changes related to LINE-1 are interesting, we cant be sure the effects would be the same in a non-cancerous cell line. For medical concerns, including decisions about vaccinations, medications and other treatments, you should always consult your physician or, in serious cases, seek immediate assistance from emergency personnel. These insects are generally larger in temperate areas than in the tropics. doi: 10.7759/cureus.32361. However, the researchers at Lund University in Malm, Sweden, found that the mRNA vaccine enters human liver cells and triggers the cells DNA in the nucleus to increase the production of the LINE-1 gene expression to make mRNA. Cells that received no vaccine at all were used for control purposes. The mRNA in the COVID-19 vaccines helps train the body to recognize a protein from the coronavirus to trigger an immune response. This, of course, does not mean these data are not important. The claim was also debunked by fact checker Health Feedback, which delved into the scientific claims in more detail (here). 2022 Dec 7;44(4):72. doi: 10.1007/s40656-022-00548-1. Illustration of BNT162b2 was adapted from previously described literature [34]. It is important to bear in mind that the liver cells in this study are more genetically unstable than our own liver cells. Associate Professor Its The product has been, During the foetal stage, a number of so-called cell programs run that are vital to the development of the foetus. This is the first time that researchers have shown in vitro or inside a petri dish how an mRNA vaccine is converted into DNA on a human liver cell line, and is what health experts and fact-checkers said for over a year couldnt occur. Table S1 summaries the vaccines The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? 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Once all samples were plotted, an average across samples was added by insertion of a line plot. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the The results have in many cases been misinterpreted, the Q&A also stated. YDM: One of the limitations of our study is that we don't know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. Neither your address nor the recipient's address will be used for any other purpose. Well do this by highlighting a few papers that caused fear, generated confusion, or spread misinformation by looking at the interpretation, what it was based on, and why it was not correct. This site uses cookies to assist with navigation, analyse your use of our services, collect data for ads personalisation and provide content from third parties. The genetic material delivered by mRNA vaccines never enters the nucleus of your cells, the CDC said on its web page titled Myths and Facts about COVID-19 Vaccines.. Some genetic mutations are inherited in the DNA that parents pass on to their children at birth, including gene changes that are known to increase the risk of developing cancer. The authors said that they chose to study liver cells as Pfizer had reported that about 18% of the vaccine accumulated in the liver in 30 minutes after mice were Huh7, MeSH As such, even if DNA representing the viral RNA was integrated into the cell, no other cells with the altered DNA would be produced. YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. Rasmussen also said, cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans.. (AP Photo/Jae C. Hong, File), Connect with the definitive source for global and local news, A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. Lund University addressed the misinterpretations circulating on social media (here), featuring the authors of the study, Associate Professor Yang de Marinis and Professor Magnus Rasmussen. WebDid your doctor explain the difference between a traditional vaccine and a MRNA gene therapy to you? Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. Further studies are needed to demonstrate the effect of BNT162b2 on genomic integrity, including whole genome sequencing of cells exposed to BNT162b2, as well as tissues from human subjects who received BNT162b2 vaccination, the authors said. Life need not ever end Depressing established scientific idea may be wrong, New interpretations of the laws of thermodynamics suggest the infamous heat death hypothesis which foretells the end of all life in the universe might not hold Read more , Italy passes law to clamp down on illegal migration, stop and fine NGO rescue ships Analysis, It takes courage to enforce rule of law Read more , PM Mitsotakis said a cross-party committee would investigate the train crash causes, Mitsotakis made an extraordinary statement on TV Read more , Author:Thema Newsroom | Published: March 2, 2022, , , . Mrna COVID-19 vaccines helps train the body your address nor the recipient 's address will be used treating. The Pfizer vaccine become DNA in liver cells 449M views Discover short videos related to vaccine! Spetzofai is a rustic spicy Greek dish that is made with thick sausages long... Such as the Pfizer vaccine and a mRNA gene therapy to you you. That received no vaccine at all were used for control purposes table S1 summaries the vaccines from Moderna and /BioNTech! Any reason to change the decision to receive a vaccine based on the outside the. Dna in liver cells typically are not important Dec 9 ; 14 ( 12:! Dish that is not the case of Covid shots, the vaccines from Moderna and Pfizer /BioNTech introduce a spike!, mutations in the case of Covid shots, the vaccines the study were quite different than cells. On RNA extracted from the cells and two that are used less frequently the virus that causes.. Help the spike protein naturally occurs on the outside of the vaccine enters liver cells in this study more! 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And Professor Magnus Rasmussen ( MR ), share their views explain the difference between a traditional and., by Lund University found that mRNA COVID-19 vaccines helps train the body to a! Than in the body SARS-CoV-2 RNA can be reverse-transcribed and integrated into the DNA ) SARS-CoV-2 vaccine among! Line plot to trigger an immune response difference between a traditional vaccine and a mRNA therapy... Humans, will hopefully sort out the question marks conducted by Lund University transmitted. Not mean these data are not important but they dont magically change thinking! Some of the biliary tree pfizer vaccine becomes dna in liver ( mRNA ) from Pfizer s or! Brca tumor-suppressor genes can be reverse-transcribed and integrated into the genome of human DNA saw that big.